In vivo functional photoacoustic micro-imaging of the electrically stimulated rat brain with multi-wavelengths

Lun De Liao*, Meng Lin Li, Hsin Yi Lai, You Yin Chen, Chang-Po Chao, Po Hsun Wang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, we report on using multi-wavelength photoacoustic microscopy to image hemodynamic changes of total hemoglobin concentration (HbT) (i.e., blood volume) and oxygenation (SO2) in rat brain cortex vessels with electrical stimulation. Electrical stimulation of the rat left forelimb was applied to evoke changes in vascular dynamics of the rat somatosensory cortex. The applied current pulses were with a pulse frequency of 3 Hz, pulse duration of 0.2 ms, and pulse amplitude of 5 mA, respectively. The imaging target of rat brains was demarcated at AP 0 - -2.5 mm and ML ± 6 mm with respect to bregma. HbT changes were probed by images acquired at 570 nm, a hemoglobin isosbestic point while SO2 changes were imaged by those acquired at 560 nm or 600 nm and their derivatives, which were normalized to those with 570 nm wavelengths. Correlation between the electrical stimulation paradigm and images acquired at 570, 560, and 600 nm in contralateral and ipsilateral vasculature was statistically analyzed, showing that the HbT and SO2 changes revealed by multi-wavelength photoacoustic images spatially correlated with contralateral vasculature.

Original languageEnglish
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2010
DOIs
StatePublished - 3 May 2010
EventPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, United States
Duration: 24 Jan 201026 Jan 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7564
ISSN (Print)1605-7422

Conference

ConferencePhotons Plus Ultrasound: Imaging and Sensing 2010
CountryUnited States
CitySan Francisco, CA
Period24/01/1026/01/10

Keywords

  • Electrical stimulation
  • Functional photoacoustic microscopy
  • Hemoglobin concentration
  • Rat's brain imaging

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    Liao, L. D., Li, M. L., Lai, H. Y., Chen, Y. Y., Chao, C-P., & Wang, P. H. (2010). In vivo functional photoacoustic micro-imaging of the electrically stimulated rat brain with multi-wavelengths. In Photons Plus Ultrasound: Imaging and Sensing 2010 [75643F] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7564). https://doi.org/10.1117/12.843085