In vitro investigation of methylene blue-bearing, electrostatically assembled aptamer-silica nanocomposites as potential photodynamic therapeutics

Tian Shyng Ding, Xin Chun Huang, Yun Ling Luo, Hsin-Yun Hsu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Photodynamic therapy, that is, excitation of a photosensitizer with light to generate reactive oxygen species such as singlet oxygen, has emerged as a noninvasive technique for cancer theranostics. However, the clinical use of many photosensitizers is impeded by their hydrophobicity, the nonspecific damage they cause to normal tissues, and their susceptibility to environmental degradation. In this study, we developed a simple electrostatic adsorption strategy to fabricate aptamer-silica nanocomposites by sequentially functionalizing nanocomposites with the cell surface-associated mucin 1 aptamer for tumor targeting and a hydrophilic photosensitizer, methylene blue, for photodynamic therapy applications. We investigated the relationship between the biophysical properties and cellular uptake of such nanocomposites to improve their formulation. Effective generation of singlet oxygen was achieved with a low photosensitizer dosage (0.5μM) and a short, low-power irradiation (1min, 10mW/cm 2 ). With the current strategy, the efficiency of photodynamic therapy was determined by the cellular uptake of nanocomposites and the targeting molecules used.

Original languageEnglish
Pages (from-to)217-224
Number of pages8
JournalColloids and Surfaces B: Biointerfaces
Volume135
DOIs
StatePublished - 1 Nov 2015

Keywords

  • Aptamer-silica nanocomposite
  • Electrostatic assembly
  • Methylene blue
  • Photodynamic therapy
  • Singlet oxygen

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