Improving antigenicity of the recombinant hepatitis C virus core protein via random mutagenesis

Chen Ji Huang, Hwei-Ling Peng*, Chih Yu Cheng

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

In order to enhance the sensitivity of diagnosis, a recombinant clone containing domain I of HCV core (amino acid residues 1 to 123) was subjected to random mutagenesis. Five mutants with higher sensitivity were obtained by colony screening of 616 mutants using reverse ELISA. Sequence analysis of these mutants revealed alterations focusing on W 84 , P 95 , P 110 , or V 129 . The inclusion bodies of these recombinant proteins overexpressed in E. coli BL21(DE3) were subsequently dissolved using 6 M urea and then refolded by stepwise dialysis. Compared to the unfolded wild-type antigen, the refolded M3b antigen (W 84 S, P 110 S and V 129 L) exhibited an increase of 66% antigenicity with binding capacity of 0.96 and affinity of 113 μM -1 . Moreover, the 33% decrease of the production demand suggests that M3b is a potential substitute for anti-HCV antibody detection.

Original languageEnglish
Article number359042
JournalJournal of Biomedicine and Biotechnology
Volume2011
DOIs
StatePublished - 24 Nov 2011

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