Improvement of axial excitation confinement in temporal focusing-based multiphoton microscopy via spatially modulated illumination

Chia Yuan Chang, Shean-Jen Chen

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement (AEC) of a few microns. Herein, a developed TFMPEM with a digital micromirror device (DMD), acting as the blazed grating for light spatial dispersion and simultaneous patterned illumination, has been extended to implement spatially modulated illumination at structured frequency and orientation. By implementing the spatially modulated illumination, the beam coverage at the back-focal aperture of the objective lens can be increased. As a result, the AEC can be condensed from 3.0 μm to 1.5 μm in full width at half maximum for a 2-fold enhancement. Furthermore, by using HiLo microscopy with two structured illuminations at the same spatial frequency but different orientation, biotissue images according to the structured illumination with condensed AEC is obviously superior in contrast and scattering suppression.

Original languageEnglish
Title of host publicationEmerging Digital Micromirror Device Based Systems and Applications IX
EditorsMichael R. Douglass, Benjamin L. Lee
PublisherSPIE
ISBN (Electronic)9781510606753
DOIs
StatePublished - 1 Jan 2017
EventEmerging Digital Micromirror Device Based Systems and Applications IX - San Francisco, United States
Duration: 30 Jan 201731 Jan 2017

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume10117
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X

Conference

ConferenceEmerging Digital Micromirror Device Based Systems and Applications IX
CountryUnited States
CitySan Francisco
Period30/01/1731/01/17

Keywords

  • digital micromirror device
  • Multiphoton processes
  • Nonlinear microscopy
  • Temporal focusing
  • Three-dimensional microscopy

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