Sulfation made easy: Syntheses of tyrosine-sulfated proteins were developed by coupling an in situ 3'-phosphoadenosine-5'-phosphosulfate (PAPS)-generating system and tyrosylprotein sulfotransferase (TPST) catalysis. High catalytic efficiency of TPST in vitro was demonstrated, and by using a similar strategy, sulfated proteins were produced in vivo through bacterial cultivation.
- Enzyme catalysis
- Post-translational modifications
- Protein modifications