Implantation of Post-translational Tyrosylprotein Sulfation into a Prokaryotic Expression System

Lu Yi Lu, Bo Han Chen, Jennifer Yun Shin Wu, Chen Chu Wang, Da Huang Chen, Yuh-Shyong Yang*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

Sulfation made easy: Syntheses of tyrosine-sulfated proteins were developed by coupling an in situ 3'-phosphoadenosine-5'-phosphosulfate (PAPS)-generating system and tyrosylprotein sulfotransferase (TPST) catalysis. High catalytic efficiency of TPST in vitro was demonstrated, and by using a similar strategy, sulfated proteins were produced in vivo through bacterial cultivation.

Original languageEnglish
Pages (from-to)377-379
Number of pages3
JournalChemBioChem
Volume12
Issue number3
DOIs
StatePublished - 11 Feb 2011

Keywords

  • Enzyme catalysis
  • Post-translational modifications
  • Protein modifications
  • Sulfation
  • Sulfotransferases

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