Immunocapture couples with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for rapid detection of type 1 dengue virus

Wen Hung Chen, I. Hsiang Hsu, Yuh Chang Sun, Yu Kuo Wang, Tung-Kung Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

A facile method for accurate detection of type 1 dengue virus (DV1) infection from complex biological mixtures, using type specific immunocapture coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was developed. The biological mixtures were treated with magnetic particles coated with a monoclonal antibody directly against type 1 dengue virus. After immunocapture purification, the DV1 was eluted with 30% acetic acid, directly spotted with seed-layer method, and analyzed by MALDI-TOF MS for DV1 capsid protein. The detection limit of the assay was ∼105pfu/mL by MALDI-TOF MS. The immunocapture could unambiguously differentiate the DV1 from other serotypes of the dengue viruses and Japanese encephalitis virus, and could be used as a specific probe to detect DV1 from complex biological mixtures.

Original languageEnglish
Pages (from-to)21-27
Number of pages7
JournalJournal of Chromatography A
Volume1288
DOIs
StatePublished - 3 May 2013

Keywords

  • Dengue virus
  • Immunocapture
  • Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
  • Monoclonal antibody

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