Immobilized rolling circle amplification on extended-gate field-effect transistors with integrated readout circuits for early detection of platelet-derived growth factor

Ming Yu Lin, Wen Yang Hsu, Yuh-Shyong Yang, Jo Wen Huang, Yueh Lin Chung, Hsin Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

Detection of tumor-related proteins with high specificity and sensitivity is important for early diagnosis and prognosis of cancers. While protein sensors based on antibodies are not easy to keep for a long time, aptamers (single-stranded DNA) are found to be a good alternative for recognizing tumor-related protein specifically. This study investigates the feasibility of employing aptamers to recognize the platelet-derived growth factor (PDGF) specifically and subsequently triggering rolling circle amplification (RCA) of DNAs on extended-gate field-effect transistors (EGFETs) to enhance the sensitivity. The EGFETs are fabricated by the standard CMOS technology and integrated with readout circuits monolithically. The monolithic integration not only avoids the wiring complexity for a large sensor array but also enhances the sensor reliability and facilitates massive production for commercialization. With the RCA primers immobilized on the sensory surface, the protein signal is amplified as the elongation of DNA, allowing the EGFET to achieve a sensitivity of 8.8 pM, more than three orders better than that achieved by conventional EGFETs. Moreover, the responses of EGFETs are able to indicate quantitatively the reaction rates of RCA, facilitating the estimation on the protein concentration. Our experimental results demonstrate that immobilized RCA on EGFETs is a useful, label-free method for early diagnosis of diseases related to low-concentrated tumor makers (e.g., PDGF) for serum sample, as well as for monitoring the synthesis of various DNA nanostructures in real time. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)4785-4797
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
Volume408
Issue number17
DOIs
StatePublished - 1 Jul 2016

Keywords

  • Amplification
  • Extended-gate field-effect transistors
  • Label-free detection
  • Monolithically integrated circuits
  • Real-time monitoring
  • Rolling circle

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