Imaging of biological tissues with pixel-level analysis of second-order susceptibility

Po-Sheng Hu, Ara Ghazaryan, Vladimir Hovhannisyan, Shean-Jen Chen, Yang Fang Chen, Chang Seok Kim, Tsung Hua Tsai*, Chen Yuan Dong

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We discuss the recent advances in the development and applications of second-order susceptibility as a contrast mechanism in optical microscopy for biological tissues. We review nonlinear optical methods and approaches for differentiation of tissue structures and discrimination of normal and pathological skin tissues, which have been demonstrated for the potential use in clinical diagnosis. In addition, the potential of secondorder susceptibility imaging, encompassing applications in differentiating various types of collagen molecules for clinical diagnosis, is demonstrated. Finally, we discuss future development and application of this technique.

Original languageEnglish
Article number031102
JournalJournal of Biomedical Optics
Issue number3
StatePublished - 15 Jul 2013


  • collagen
  • polarization-resolved microscopy
  • second harmonic generation
  • second-order susceptibility

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