Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy

Lun De Liao, Meng Lin Li, Hsin Yi Lai, Yen Yu I. Shih, Yu Chun Lo, Siny Tsang, Paul C.-P. Chao, Chin Teng Lin, Fu Shan Jaw, You Yin Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

The present study reported the development of a novel functional photoacoustic microscopy (fPAM) system for investigating hemodynamic changes in rat cortical vessels associated with electrical forepaw stimulation. Imaging of blood optical absorption by fPAM at multiple appropriately-selected and distinct wavelengths can be used to probe changes in total hemoglobin concentration (HbT, i.e., cerebral blood volume [CBV]) and hemoglobin oxygen saturation (SO2). Changes in CBV were measured by images acquired at a wavelength of 570nm (Λ570), an isosbestic point of the molar extinction spectra of oxy- and deoxy-hemoglobin, whereas SO2 changes were sensed by pixel-wise normalization of images acquired at Λ560 or Λ600 to those at Λ570. We demonstrated the capacity of the fPAM system to image and quantify significant contralateral changes in both SO2 and CBV driven by electrical forepaw stimulation. The fPAM system complements existing imaging techniques, with the potential to serve as a favorable tool for explicitly studying brain hemodynamics in animal models.

Original languageEnglish
Pages (from-to)562-570
Number of pages9
JournalNeuroImage
Volume52
Issue number2
DOIs
StatePublished - 1 Aug 2010

Keywords

  • Electrical stimulation
  • Functional photoacoustic microscopy (fPAM)
  • Hemoglobin oxygen saturation (SO)
  • Total hemoglobin concentration (HbT)

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    Liao, L. D., Li, M. L., Lai, H. Y., Shih, Y. Y. I., Lo, Y. C., Tsang, S., Chao, P. C-P., Lin, C. T., Jaw, F. S., & Chen, Y. Y. (2010). Imaging brain hemodynamic changes during rat forepaw electrical stimulation using functional photoacoustic microscopy. NeuroImage, 52(2), 562-570. https://doi.org/10.1016/j.neuroimage.2010.03.065