Identification of hepatocellular carcinomas with contrast enhanced 40 MHz ultrasound in hepatitis B virus X protein transgenic mice

Ai Ho Liao*, Yun-Chien Cheng, Shiou Hwei Yeh, Pai Chi Li

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations


Contrast-enhanced ultrasound (CEUS) imaging is a reliable noninvasive clinical method of detecting and characterizing hepatocellular carcinoma (HCC) lesions. In this study, high-frequency (40 MHz) CEUS imaging was applied to small animals. Specifically, high-frequency ultrasound liver imaging with albumin-shelled microbubbles was employed to characterize suspected HCC lesions that developed in five Hepatitis B virus X (HBx) transgenic mice at around 14 to 16 months of age. After injecting the contrast agent, the time-intensity curves (TICs) of suspected HCC lesions, vessels in suspected HCC lesions, and surrounding liver parenchyma tissues were measured for 30 minutes. In four of the five mice, the intensity of the TICs of the suspected HCC lesions was enhanced by a mean of 13.5 dB in the arterial phase (at around 10-30 seconds), with a rapid "washout" starting at the beginning of the portal phase. The peak intensity relative to the baseline also increased from 3.27 dB at 18 months to 23.74 dB at 22 months. On the other hand, the mean contrast between the suspected HCC lesion and the liver parenchyma increased by 6.6 dB, thus allowing clear detection of the HCC margin. Histopathology investigations confirmed the HCC development in these four mice. For the remaining mouse, the absence of the peak in the arterial phase was also consistent with the histopathology finding of dysplasia. These results demonstrated that high-frequency CEUS imaging is an effective preclinical tool for identifying HCC lesions in mice.

Original languageEnglish
Title of host publication2007 IEEE Ultrasonics Symposium Proceedings, IUS
Number of pages4
StatePublished - 1 Dec 2007
Event2007 IEEE Ultrasonics Symposium, IUS - New York, NY, United States
Duration: 28 Oct 200731 Oct 2007

Publication series

NameProceedings - IEEE Ultrasonics Symposium
ISSN (Print)1051-0117


Conference2007 IEEE Ultrasonics Symposium, IUS
CountryUnited States
CityNew York, NY

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