Identification and characterization of the nuclear import and export signals of the mammalian Ste20-like protein kinase 3

Wan Shu Lee, Chiung Yueh Hsu, Pei Ling Wang, Chi Ying Fred Huang, Chia Hua Chang, Chiun-Jye Yuan*

*Corresponding author for this work

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

Mst3, a human Ste20-like protein kinase, has been recently demonstrated to undergo a caspase-mediated cleavage during apoptosis. The proteolytic cleavage of the C-terminus of Mst3 caused nuclear translocation of its kinase domain. This work provides evidence that Mst3 may contain a bipartite-like nuclear localization sequence (NLS) at the C-terminus of its kinase domain (residues 278-292). The removal of NLS from the kinase domain of Mst3 led to the cytoplasmic accumulation of EGFP-Mst3 Δ277. The presence of nuclear exporting signals in the Mst3 was also demonstrated by leptomycin B-treatment and serial deletion of the C-terminal regulatory domain of Mst3. A nuclear export signal was also postulated to be in the regions of amino acids 335-386. In conclusion, Mst3 contains both NLS and NES signals, which may cooperate to control the subcellular distribution of Mst3.

Original languageEnglish
Pages (from-to)41-45
Number of pages5
JournalFEBS Letters
Volume572
Issue number1-3
DOIs
StatePublished - 13 Aug 2004

Keywords

  • GFP, green fluorescence protein
  • LMB, leptomycin B
  • Mst, mammalian Ste20-like protein kinase
  • Mst3
  • NES, nuclear exporting signal
  • NLS, nuclear localization sequence
  • Nuclear exporting signal
  • Nuclear localization sequence
  • Nuclear trafficking
  • Ste20

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