Identification and characterization of KvgAS, a two-component system in Klebsiella pneumoniae CG43

Yi Chyi Lai, Ging Ting Lin, Shu Li Yang, Hwan You Chang, Hwei-Ling Peng*

*Corresponding author for this work

Research output: Contribution to journalArticle

14 Scopus citations

Abstract

A two-component system encoding gene cluster kvgAS that is present only in virulent Klebsiella pneumoniae CG43 was isolated and its sequence determined. RT-PCR and Southern analysis demonstrated that kvgAS is organized as an operon. No apparent effect of a kvgS deletion on bacterial virulence was observed in a mouse peritonitis model. In the presence of paraquat or 2,2-dipyridyl, the activity of kvgAS promoter in the kvgS mutant was found to be reduced to half of the level in the wild-type strain. The data suggest that the KvgAS system is autoregulated and plays a role in countering free radical stresses and sensing iron-limiting conditions.

Original languageEnglish
Pages (from-to)121-126
Number of pages6
JournalFEMS Microbiology Letters
Volume218
Issue number1
DOIs
StatePublished - 21 Jan 2003

Keywords

  • Histidine kinase
  • Klebsiella gene
  • Response regulator
  • Two-component system

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