Hypochlorous acid turn-on boron dipyrromethene probe based on oxidation of methyl phenyl sulfide

Shi Rong Liu, Mani Vedamalai, Shu-Pao Wu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

A boron dipyrromethene (BODIPY)-based fluorometric probe, HCS, has been successfully developed for the highly sensitive and selective detection of hypochlorous acid (HOCl). The probe is based on the specific HOCl-promoted oxidation of methyl phenyl sulfide. The reaction is accompanied by a 160-fold increase in the fluorescent quantum yield (from 0.003 to 0.480). The fluorescent turn-on mechanism is accomplished by suppression of photoinduced electron transfer (PET) from the methyl phenyl sulfide group to BODIPY. The fluorescence intensity of the reaction between HOCl and HCS shows a good linearity in the HOCl concentration range 1-10. μM. The detection limit is 23.7. nM (S/N. = 3). In addition, confocal fluorescence microscopy imaging using RAW264.7 macrophages demonstrates that the HCS probe could be an efficient fluorescent detector for HOCl in living cells.

Original languageEnglish
Pages (from-to)71-76
Number of pages6
JournalAnalytica Chimica Acta
Volume800
DOIs
StatePublished - 24 Oct 2013

Keywords

  • Bioimaging
  • Boron dipyrromethene
  • Fluorescent probe
  • Hypochlorous acid

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