AR & VR display technology had been developed and expanded rapidly in recent years. Human factors in 3D depth perception right then becomes a very important issue. Because the human vision response in AR & VR is definitely different from stereoptic images created by human macula of the real world.Macula is in the center of retina which create most important vision for human brain to recognize the images. During the general eye usage, macula in not so important for wide field image. However, macula will receive heavy light dosage when playing AR & VR. Therefore, under the responded command of macula, ciliary muscle contraction will help to precise focusing on macula. Under using AR & VR for a long period, macula will over receive the blue light emissive by the display of AR/VR, and also the ciliary muscle spasm will cause eye strain and pseudomyopia. However, the pupil constriction during near vision will lower the eye pressure, thus can help release the eye fatigue when watching AR & VR. According to the previous vision analysis, the index of physiological measurement for visual response should be developed and standardized in order to improve the AR & VR display in the future.