Three new pyrene derivatives (MS1, MS2, and MS3) containing two triazole units exhibited fluorescence quenching in the presence of Hg2+ ions. Other metal ions Ag+, Ca2+, Cd2+, Co 2+, Cu2+, Fe2+, Fe3+, K+, Mg2+, Mn2+, Na+, Ni2+, Pb 2+, Rb+, and Zn2+ produced only minor changes in the fluorescence of MS1, MS2, and MS3. The binding ratio of the chemosensor-Hg2+ complexes was found to be 1:1 according to Job plot experiments. The association constants (Ka) of chemosensor-Hg 2+ complexes were found to be 1.68×103 M -1 (MS1), 1.57×103 M-1 (MS2), and 1.52×103 M-1 (MS3), respectively. Moreover, fluorescence microscopy experiments showed that MS1 could be used as a fluorescent probe for detecting Hg2+ in living cells.
- Imaging agents