Highly fluorescent probe for copper (II) ion based on commercially available compounds and live cell imaging

Duraisamy Udhayakumari, Sivan Velmathi*, Yi Ming Sung, Shu-Pao Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

62 Scopus citations

Abstract

The commercially available 2, 3-diaminophenazine, 1, 2-diamino- anthraquinone and 2, 4-dinitrophenylhydrazine (S1-S3) act as a colorimetric, fluorescent probes for selective detection of Cu2+ ions in aqueous medium over other metal ions. In presence of Cu2+, S1 shows fluorescent turn-off by the paramagnetic effect. S2 and S3 with Cu2+ ions show fluorescent turn-on due to the inhibition of photoinduced electron transfer mechanism. The fluorescent probes S1-S3 detect Cu2+ ions in aqueous solution at nanomolar levels. Theoretical calculations were employed to understand the sensing mechanism of the sensors towards Cu2+. S1, S2 and S3 were further applied for biological imaging to confirm that it can be used as a fluorescent probe for monitoring Cu2+ in living cells, and demonstrated its value in practical applications such as environmental and biological systems.

Original languageEnglish
Pages (from-to)285-293
Number of pages9
JournalSensors and Actuators, B: Chemical
Volume198
DOIs
StatePublished - 31 Jul 2014

Keywords

  • Aqueous solution
  • Bioimaging
  • Copper ion
  • Paramagnetic effect
  • PET
  • Selectivity

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