High-throughput multiphoton-induced threedimensional ablation and imaging for biotissues

Chun Yu Lin, Pei Kao Li, Li Chung Cheng, Yi Cheng Li, Chia Yuan Chang, Ann Shyn Chiang, Chen Yuan Dong, Shean Jen Chen*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

In this study, a temporal focusing-based high-throughput multiphoton-induced ablation system with axially-resolved widefield multiphoton excitation has been successfully applied to rapidly disrupt biotissues. Experimental results demonstrate that this technique features high efficiency for achieving large-area laser ablation without causing serious photothermal damage in non-ablated regions. Furthermore, the rate of tissue processing can reach around 1.6 × 106 μm3/s in chicken tendon. Moreover, the temporal focusing-based multiphoton system can be efficiently utilized in optical imaging through iterating high-throughput multiphoton-induced ablation machining followed by widefield optical sectioning; hence, it has the potential to obtain molecular images for a whole bio-specimen.

Original languageEnglish
Pages (from-to)491-499
Number of pages9
JournalBiomedical Optics Express
Volume6
Issue number2
DOIs
StatePublished - 1 Jan 2015

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