High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors

Ming Yu Lin; Yu Wei Chang; Yu Ting Tai; Yuh-Shyong Yang; Yang Tung Huang

doi:10.1109/ICSENST.2008.4757079

High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors

Ming Yu Lin, Yu Wei Chang, Yu Ting Tai, Yuh-Shyong Yang, Yang Tung Huang

Department of Biological Science and Technology

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

1 Scopus citations

Abstract

Colorimetric, in situ, isothermal rolling circle amplification (RCA)-based biosensor was developed as a novel, versatile nucleic acid-based amplification machine for platelet-derived growth factor (PDGF) detection. This antibody-free biosensor utilized nucleic acids immobilized on the plate and DNA aptamers to capture the target protein. As compared to the conventional fluorescence measurement [1], the reported colorimetic method can facilitate the development of RCA biosensors in a high sensitive, cost-less optoelectronic detection system. Our data indicates that the colorimetric RCA assay was able to detect PDGF 0.32 nM and showed a good linear correlation between PDGF concentration and absorbance with correlation coefficient of 0.99.

Original language	English
Title of host publication	Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008
Pages	86-89
Number of pages	4
DOIs	https://doi.org/10.1109/ICSENST.2008.4757079
State	Published - 1 Dec 2008
Event	3rd International Conference on Sensing Technology, ICST 2008 - Tainan, Taiwan Duration: 30 Nov 2008 → 3 Dec 2008

Publication series

Name	Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008

Conference

Conference	3rd International Conference on Sensing Technology, ICST 2008
Country	Taiwan
City	Tainan
Period	30/11/08 → 3/12/08

Keywords

Aptamer
Colorimetric
PDGF
Rolling circle amplification

Access to Document

10.1109/ICSENST.2008.4757079

Link to publication in Scopus

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Lin, M. Y., Chang, Y. W., Tai, Y. T., Yang, Y-S., & Huang, Y. T. (2008). High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors. In Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008 (pp. 86-89). [4757079] (Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008). https://doi.org/10.1109/ICSENST.2008.4757079

Lin, Ming Yu ; Chang, Yu Wei ; Tai, Yu Ting ; Yang, Yuh-Shyong ; Huang, Yang Tung. / High sensitive, colorimetric, isothermal nucleic acids amplification : A versatile platform for protein biosensors. Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008. 2008. pp. 86-89 (Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008).

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title = "High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors",

abstract = "Colorimetric, in situ, isothermal rolling circle amplification (RCA)-based biosensor was developed as a novel, versatile nucleic acid-based amplification machine for platelet-derived growth factor (PDGF) detection. This antibody-free biosensor utilized nucleic acids immobilized on the plate and DNA aptamers to capture the target protein. As compared to the conventional fluorescence measurement [1], the reported colorimetic method can facilitate the development of RCA biosensors in a high sensitive, cost-less optoelectronic detection system. Our data indicates that the colorimetric RCA assay was able to detect PDGF 0.32 nM and showed a good linear correlation between PDGF concentration and absorbance with correlation coefficient of 0.99.",

keywords = "Aptamer, Colorimetric, PDGF, Rolling circle amplification",

author = "Lin, {Ming Yu} and Chang, {Yu Wei} and Tai, {Yu Ting} and Yuh-Shyong Yang and Huang, {Yang Tung}",

year = "2008",

month = dec,

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doi = "10.1109/ICSENST.2008.4757079",

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isbn = "9781424421770",

series = "Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008",

pages = "86--89",

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note = "null ; Conference date: 30-11-2008 Through 03-12-2008",

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Lin, MY, Chang, YW, Tai, YT, Yang, Y-S & Huang, YT 2008, High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors. in Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008., 4757079, Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008, pp. 86-89, 3rd International Conference on Sensing Technology, ICST 2008, Tainan, Taiwan, 30/11/08. https://doi.org/10.1109/ICSENST.2008.4757079

High sensitive, colorimetric, isothermal nucleic acids amplification : A versatile platform for protein biosensors. / Lin, Ming Yu; Chang, Yu Wei; Tai, Yu Ting; Yang, Yuh-Shyong; Huang, Yang Tung.

Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008. 2008. p. 86-89 4757079 (Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

TY - GEN

T1 - High sensitive, colorimetric, isothermal nucleic acids amplification

AU - Lin, Ming Yu

AU - Chang, Yu Wei

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N2 - Colorimetric, in situ, isothermal rolling circle amplification (RCA)-based biosensor was developed as a novel, versatile nucleic acid-based amplification machine for platelet-derived growth factor (PDGF) detection. This antibody-free biosensor utilized nucleic acids immobilized on the plate and DNA aptamers to capture the target protein. As compared to the conventional fluorescence measurement [1], the reported colorimetic method can facilitate the development of RCA biosensors in a high sensitive, cost-less optoelectronic detection system. Our data indicates that the colorimetric RCA assay was able to detect PDGF 0.32 nM and showed a good linear correlation between PDGF concentration and absorbance with correlation coefficient of 0.99.

AB - Colorimetric, in situ, isothermal rolling circle amplification (RCA)-based biosensor was developed as a novel, versatile nucleic acid-based amplification machine for platelet-derived growth factor (PDGF) detection. This antibody-free biosensor utilized nucleic acids immobilized on the plate and DNA aptamers to capture the target protein. As compared to the conventional fluorescence measurement [1], the reported colorimetic method can facilitate the development of RCA biosensors in a high sensitive, cost-less optoelectronic detection system. Our data indicates that the colorimetric RCA assay was able to detect PDGF 0.32 nM and showed a good linear correlation between PDGF concentration and absorbance with correlation coefficient of 0.99.

KW - Aptamer

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KW - PDGF

KW - Rolling circle amplification

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Lin MY, Chang YW, Tai YT, Yang Y-S, Huang YT. High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors. In Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008. 2008. p. 86-89. 4757079. (Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008). https://doi.org/10.1109/ICSENST.2008.4757079