High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors

Ming Yu Lin, Yu Wei Chang, Yu Ting Tai, Yuh-Shyong Yang, Yang Tung Huang

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

Colorimetric, in situ, isothermal rolling circle amplification (RCA)-based biosensor was developed as a novel, versatile nucleic acid-based amplification machine for platelet-derived growth factor (PDGF) detection. This antibody-free biosensor utilized nucleic acids immobilized on the plate and DNA aptamers to capture the target protein. As compared to the conventional fluorescence measurement [1], the reported colorimetic method can facilitate the development of RCA biosensors in a high sensitive, cost-less optoelectronic detection system. Our data indicates that the colorimetric RCA assay was able to detect PDGF 0.32 nM and showed a good linear correlation between PDGF concentration and absorbance with correlation coefficient of 0.99.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Conference on Sensing Technology, ICST 2008
Pages86-89
Number of pages4
DOIs
StatePublished - 1 Dec 2008
Event3rd International Conference on Sensing Technology, ICST 2008 - Tainan, Taiwan
Duration: 30 Nov 20083 Dec 2008

Publication series

NameProceedings of the 3rd International Conference on Sensing Technology, ICST 2008

Conference

Conference3rd International Conference on Sensing Technology, ICST 2008
CountryTaiwan
CityTainan
Period30/11/083/12/08

Keywords

  • Aptamer
  • Colorimetric
  • PDGF
  • Rolling circle amplification

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