@inproceedings{ead8799ae87241cdb9fd92547ef0eb39,
title = "High sensitive, colorimetric, isothermal nucleic acids amplification: A versatile platform for protein biosensors",
abstract = "Colorimetric, in situ, isothermal rolling circle amplification (RCA)-based biosensor was developed as a novel, versatile nucleic acid-based amplification machine for platelet-derived growth factor (PDGF) detection. This antibody-free biosensor utilized nucleic acids immobilized on the plate and DNA aptamers to capture the target protein. As compared to the conventional fluorescence measurement [1], the reported colorimetic method can facilitate the development of RCA biosensors in a high sensitive, cost-less optoelectronic detection system. Our data indicates that the colorimetric RCA assay was able to detect PDGF 0.32 nM and showed a good linear correlation between PDGF concentration and absorbance with correlation coefficient of 0.99.",
keywords = "Aptamer, Colorimetric, PDGF, Rolling circle amplification",
author = "Lin, {Ming Yu} and Chang, {Yu Wei} and Tai, {Yu Ting} and Yuh-Shyong Yang and Huang, {Yang Tung}",
year = "2008",
month = dec,
day = "1",
doi = "10.1109/ICSENST.2008.4757079",
language = "English",
isbn = "9781424421770",
series = "Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008",
pages = "86--89",
booktitle = "Proceedings of the 3rd International Conference on Sensing Technology, ICST 2008",
note = "null ; Conference date: 30-11-2008 Through 03-12-2008",
}