We demonstrate a dynamic light-induced optoelectroosmosis flow design for high efficiency E. coli concentration on an organic photoconductive material based biochip. Several traditional approaches of bacteria purification and enrichment utilize magnetic beads coated with antibodies to attract them. The efficiency of collecting magnetic beads is equal to sort bacteria. Our work provides a direct method to enrich the E. coli concentration with dynamic light images. Due to the applications of photoconductive material, TiOPc, two features are demonstrated, simple biochip fabrication processes and rapid E. coli collection. The biochip is fabricated only with spin-coating a thin TiOPc layer, 500nm, on the indium tin oxide (ITO) conductive glass. Besides, the E. coli are concentrated together within 30 sec toward the illuminating region by the surface charge slip. We also calibrate the dominating effects in different applied AC frequency region. In conclusion, our light-induced E. coli manipulation approach would expand the scope of the examination efficiency, for example, water quality.