High efficiency E. coli concentration via dynamic light-induced optoelectro-osmosis flow on organic photoconductivity biochip

Shih Mo Yang*, C. Y. Lin, T. P. Wang, T. H. Punde, H. Y. Chang, Long Hsu, C. H. Liu

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We demonstrate a dynamic light-induced optoelectroosmosis flow design for high efficiency E. coli concentration on an organic photoconductive material based biochip. Several traditional approaches of bacteria purification and enrichment utilize magnetic beads coated with antibodies to attract them. The efficiency of collecting magnetic beads is equal to sort bacteria. Our work provides a direct method to enrich the E. coli concentration with dynamic light images. Due to the applications of photoconductive material, TiOPc, two features are demonstrated, simple biochip fabrication processes and rapid E. coli collection. The biochip is fabricated only with spin-coating a thin TiOPc layer, 500nm, on the indium tin oxide (ITO) conductive glass. Besides, the E. coli are concentrated together within 30 sec toward the illuminating region by the surface charge slip. We also calibrate the dominating effects in different applied AC frequency region. In conclusion, our light-induced E. coli manipulation approach would expand the scope of the examination efficiency, for example, water quality.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Pages512-515
Number of pages4
StatePublished - 23 Nov 2011
EventNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 - Boston, MA, United States
Duration: 13 Jun 201116 Jun 2011

Publication series

NameTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Volume2

Conference

ConferenceNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
CountryUnited States
CityBoston, MA
Period13/06/1116/06/11

Keywords

  • Dielectrophoresis
  • E. coli
  • Electroosmosis
  • Photoconductivity
  • TiOPc

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    Yang, S. M., Lin, C. Y., Wang, T. P., Punde, T. H., Chang, H. Y., Hsu, L., & Liu, C. H. (2011). High efficiency E. coli concentration via dynamic light-induced optoelectro-osmosis flow on organic photoconductivity biochip. In Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 (pp. 512-515). (Technical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011; Vol. 2).