Herb-drug interaction of Epimedium extract on the pharmacokinetic of dapoxetine in rats

Thomas Y. Hsueh, Jing Kai Ho, Lie Chwen Lin, Allen W. Chiu, Chi Hung Lin, Tung Hu Tsai*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The aim of study is to develop a high performance liquid chromatography tandem mass spectrometry (LC-MS/MS) method to investigate the pharmacokinetic interaction of Epimedium extract on the dapoxetine in rats. Experimental rats were divided into the following four parallel groups: (1) dapoxetine alone (10 mg/kg, i.v.); (2) oral administration of Epimedium extract (2 g/kg) for 3 consecutive days and on the fourth day dapoxetine was administered (10 mg/kg, i.v.); (3) dapoxetine alone (10 mg/kg, p.o.); (4) oral administration of Epimedium extract (2 g/kg) for 3 consecutive days and on the fourth day dapoxetine was administered (10 mg/kg, p.o.). The calibration curves of dapoxetine were acquired over a concentration ranges from 1 to 500 ng/mL with the R2 = 0.999. The mean matrix effects and extraction recoveries of dapoxetine at three different concentrations (1, 10, 500 ng/mL) ranged from 107.3 to 110.9% and from 25.5 to 28.2% respectively. The interday and intraday relative standard deviation were both <6% while the bias were both <14%. The pharmacokinetic results demonstrated that pretreated with/without Epimedium extract for three consecutive days did not significant alter the pharmacokinetics of dapoxetine in rats. The oral bioavailability of dapoxetine was about 75% in rats.

Original languageEnglish
Pages (from-to)64-69
Number of pages6
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume1014
DOIs
StatePublished - 1 Mar 2016

Keywords

  • Dapoxetine
  • Epimedium sagittatum extract
  • Herbal drug interaction
  • Pharmacokinetics
  • Traditional Chinese medicine

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