Heavy chain of cytoplasmic dynein is a major component of the postsynaptic density fraction

Huei Hsuan Cheng, Szu Heng Liu, Hui Cheng Lee, Ya Shiuan Lin, Zu Han Huang, Cheng I. Hsu, Yu-Chie Chen, Yen Chung Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


A protein with an apparent molecular size of 490 kDa was found in the postsynaptic density (PSD) fraction isolated from porcine cerebral cortices and rat forebrains, and this 490 kDa protein accounted for ∼3% of the total protein of these samples. Matrix-assisted laser desorption ionization-time of flight mass spectrometric and Western blotting analyses consistently indicated that this 490 kDa protein consisted primarily of the heavy chain of cytoplasmic dynein (cDHC). Immunocytochemical analyses showed that cDHC was found in 92% and 89% of the phalloidin-positive protrusions that were themselves associated with discrete clusters of synaptophysin, a presynaptic terminal marker, and PSD-95, a postsynaptic marker, on neuronal processes, respectively. Quantitative Western blotting analyses of various subcellular fractions isolated from porcine cerebral cortices and rat forebrains further showed that not only the heavy but also the intermediate chains of dynein are enriched in the PSD fraction. Cytoplasmic dynein is a microtubule-associated motor protein complex that drives the movement of various cargos toward the minus ends of microtubules and plays many other diverse functions in the cell. Our results that cDHC is a major component of the PSD fraction, that both dynein heavy and intermediate chains are enriched in the PSD fraction and that cDHC is present in dendritic spines raise the possibilities that cytoplasmic dynein may play structural and functional roles in the postsynaptic terminal.

Original languageEnglish
Pages (from-to)244-254
Number of pages11
JournalJournal of Neuroscience Research
Issue number2
StatePublished - 1 Aug 2006


  • Dendritic spines
  • Subcellular fractions

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