Growth rate regulation of lac operon expression in Escherichia coli is cyclic AMP dependent

Jong Tar Kuo, Yu Jen Chang, Ching-Ping Tseng*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

In contrast to the ribosomal RNA gene expression increasing with growth rate, transcription of the lac operon is downregulated by cell growth rate. In continuous culture, growth rate regulation of lac promoter was independent of carbon substrate used and its location on the chromosome. Since the lac operon is activated by cyclic adenosine monophosphate (cAMP), which decreases with increasing cell growth rate, expression of plac-lacZ reporter fusion was analyzed in cya mutant under various growth conditions. The results demonstrated that expression of plac-lacZ in cya mutant was both lower and growth rate independent. In addition, ppGpp (guanosine tetraphosphate) was not involved in the mechanism of growth rate regulation of the lac promoter. Thus, the results of this study indicate that cAMP mediates the growth rate-dependent regulation of lac operon expression in Escherichia coli.

Original languageEnglish
Pages (from-to)397-402
Number of pages6
JournalFEBS Letters
Volume553
Issue number3
DOIs
StatePublished - 23 Oct 2003

Keywords

  • Cyclic adenosine monophosphate
  • Escherichia coli
  • Growth rate
  • lac operon

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