Gravity-based precise cell manipulation system enhanced by in-phase mechanism

Koji Mizoue, Manh Hao Phan, Chia-Hung Tsai*, Makoto Kaneko, Junsu Kang, Wan Kyun Chung

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

This paper proposes a gravity-based system capable of generating high-resolution pressure for precise cell manipulation or evaluation in a microfluidic channel. While the pressure resolution of conventional pumps for microfluidic applications is usually about hundreds of pascals as the resolution of their feedback sensors, precise cell manipulation at the pascal level cannot be done. The proposed system successfully achieves a resolution of 100 millipascals using water head pressure with an in-phase noise cancelation mechanism. The in-phase mechanism aims to suppress the noises from ambient vibrations to the system. The proposed pressure system is tested with a microfluidic platform for pressure validation. The experimental results show that the in-phase mechanism effectively reduces the pressure turbulence, and the pressure-driven cell movement matches the theoretical simulations. Preliminary experiments on deformability evaluation with red blood cells under incremental pressures of one pascal are successfully performed. Different deformation patterns are observed from cell to cell under precise pressure control.

Original languageEnglish
Article number116
JournalMicromachines
Volume7
Issue number7
DOIs
StatePublished - 9 Jul 2016

Keywords

  • Cell deformability
  • Cell manipulation
  • Microfluidics
  • Pressure control

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