Genetic requirements for Klebsiella pneumoniae-induced liver abscess in an oral infection model

Ya Chun Tu, Min Chi Lu, Ming Ko Chiang, Shu Ping Huang, Hwei-Ling Peng, Hwan You Chang, Ming Shiou Jan, Yi Chyi Lai*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

53 Scopus citations


Klebsiella pneumoniae is the predominant pathogen of primary liver abscess. However, our knowledge regarding the molecular basis of how K. pneumoniae causes primary infection in the liver is limited. We established an oral infection model that recapitulated the characteristics of liver abscess and conducted a genetic screen to identify the K. pneumoniae genes required for the development of liver abscess in mice. Twenty-eight mutants with attenuated growth in liver or spleen samples out of 2,880 signature-tagged mutants that produced the wild-type capsule were identified, and genetic loci which were disrupted in these mutants were identified to encode products with roles in cellular metabolism, adhesion, transportation, gene regulation, and unknown functions. We further evaluated the virulence attenuation of these mutants in independent infection experiments and categorized them accordingly into three classes. In particular, the class I and II mutant strains exhibited significantly reduced virulence in mice, and most of these strains were not detected in extraintestinal tissues at 48 h after oral inoculation. Interestingly, the mutated loci of about one-third of the class I and II mutant strains encode proteins with regulatory functions, and the transcript abundances of many other genes identified in the same screen were markedly changed in these regulatory mutant strains, suggesting a requirement for genetic regulatory networks for translocation of K. pneumoniae across the intestinal barrier. Furthermore, our finding that preimmunization with certain class I mutant strains protected mice against challenge with the wild-type strain implied a potential application for these strains in prophylaxis against K. pneumoniae infections.

Original languageEnglish
Pages (from-to)2657-2671
Number of pages15
JournalInfection and Immunity
Issue number7
StatePublished - 1 Jul 2009

Fingerprint Dive into the research topics of 'Genetic requirements for Klebsiella pneumoniae-induced liver abscess in an oral infection model'. Together they form a unique fingerprint.

Cite this