General concerns of FLIM data representation and analysis: Frequency-domain model-free analysis

Yi-Chun Chen, Bryan Q. Spring, Chittanon Buranachai, Bianca Tong, George Malachowski, Robert M. Clegg

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

In the first part of this chapter we develop several mathematical expressions describing fluorescence lifetime measurements, and show the close correspondence between the time and frequency domains (Lakowicz 1999; Valeur 2002, 2005). We then discuss how the underlying physical parameters are deciphered from the measured FLIM experiments and present some techniques of image analysis and display that are peculiar to FLIM.

Original languageEnglish
Title of host publicationFlim Microscopy in Biology and Medicine
PublisherCRC Press
Pages291-399
Number of pages109
ISBN (Electronic)9781420078916
ISBN (Print)9781420078909
StatePublished - 1 Jan 2009

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