In the first part of this chapter we develop several mathematical expressions describing fluorescence lifetime measurements, and show the close correspondence between the time and frequency domains (Lakowicz 1999; Valeur 2002, 2005). We then discuss how the underlying physical parameters are deciphered from the measured FLIM experiments and present some techniques of image analysis and display that are peculiar to FLIM.
|Title of host publication||Flim Microscopy in Biology and Medicine|
|Number of pages||109|
|State||Published - 1 Jan 2009|