Functional transcranial photoacoustic micro-imaging of mouse cerebrovascular cross-section and hemoglobin oxygenation changes during forepaw electrical stimulation

Lun-De Liao, You-Yin Chen, Chin-Teng Lin, Jyh-Yeong Chang, Meng Lin Li

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

In this study, we report on using a 50-MHz functional photoacoustic microscopy (PAM) to transcranially image the cross-section and hemoglobin oxygenation (SO2) changes of single mouse cortical vessels in response to left forepaw electrical stimulation. Three difference levels of the cortical vessels (i.e., with different-sized diameters of 350, 100 and 55 mu m) on activated regions were marked to measure their functional cross-section and SO2 changes as a function of time. Electrical stimulation of the mouse left forelimb was applied to evoke functional changes in vascular dynamics of the mouse somatosensory cortex. The applied current pulses were with a pulse frequency of 3 Hz, pulse duration of 0.2 ms, and pulse amplitude of 2 mA. The cerebrovascular cross-section changes, which indicate changes in cerebral blood volume (CBV), were probed by images acquired at 570 nm, a hemoglobin isosbestic point, while SO2 changes were monitored by the derivatives of 560-nm images normalized to 570-nm ones. The results show that vessel diameter and SO2 were significantly dilated and increased when compared with those of the controlled ones. In summary, the PAM shows its promise as a new imaging modality for transcranially functional quantification of single vessel diameter (i.e., CBV) and SO2 changes without any contrast agents applied during stimulation.
Original languageEnglish
Title of host publicationPHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2011
PublisherSPIE-INT SOC OPTICAL ENGINEERING
Volume7899
ISBN (Print)978-0-8194-8436-9
DOIs
StatePublished - 2011

Keywords

  • Functional imaging
  • photoacoustic microscopy
  • electrical stimulation
  • somatosensory cortex
  • vasodilatation
  • hemoglobin oxygenation

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