Functional photoacoustic micro-imaging of rat cerebral hemodynamic response function in single vessels during forepaw electrical stimulation

Lun-De Liao*, You-Yin Chen, Chin-Teng Lin, Jyh-Yeong Chang, Meng-Lin Li

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The specificity of the hemodynamic response function (HRF) is determined spatially by the vascular architecture and temporally by the evolution of hemodynamic changes. Here, we used functional photoacoustic microscopy (fPAM) to investigate the spatiotemporal evolution of the HRFs of hemoglobin concentration (HbT), cerebral blood volume (CBV) and hemoglobin oxygen saturation (SO2) in single cerebral vessels to rat left-forepaw stimulation. The HRF changes in specific cerebral vessels responding to different stimulation intensities and durations were bilaterally imaged with 36 x 65-mu m spatial resolution. Various electrical stimulations were applied with stimulation intensities at 1, 2, 6 and 10-mA combined with 5-s and 15-s stimulation durations, respectively. Our main findings were as follows: 1) the functional HbT and SO2 increased sub-linearly with increasing stimulus intensities and 2) the results suggested that the CBV changes are more linearly correlated with arterioles than HbT and SO2 within a limited dynamic range of stimulation intensities and duration. The findings in this study indicate that the regulation of hemodynamic changes in single cerebral vessels can be reliable studied by the fPAM technique without the use of contrast agents.
Original languageEnglish
Title of host publicationPHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2012
Volume8223
DOIs
StatePublished - 2012
EventConference on Photons Plus Ultrasound - Imaging and Sensing/Photonics West BiOS Symposium - San Francisco, United States
Duration: 22 Jan 201224 Jan 2012

Conference

ConferenceConference on Photons Plus Ultrasound - Imaging and Sensing/Photonics West BiOS Symposium
CountryUnited States
CitySan Francisco
Period22/01/1224/01/12

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  • Cite this

    Liao, L-D., Chen, Y-Y., Lin, C-T., Chang, J-Y., & Li, M-L. (2012). Functional photoacoustic micro-imaging of rat cerebral hemodynamic response function in single vessels during forepaw electrical stimulation. In PHOTONS PLUS ULTRASOUND: IMAGING AND SENSING 2012 (Vol. 8223). [ 822334] https://doi.org/10.1117/12.908131