Fluorescence lifetime imaging comes of age how to do it and how to interpret it

Yi-Chun Chen, Bryan Q. Spring, Robert M. Clegg*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

7 Scopus citations


Fluorescence lifetime imaging (FLI) has been used widely for measuring biomedical samples. Practical guidelines on taking successful FLI data are provided to avoid common errors that arise during the measurement. Several methods for analyzing and interpreting FLI results are also introduced; e.g., a model-free data analysis method called the polar plot allows visualization and analysis of FLI data without iterative fitting, and an image denoising algorithm called variance-stabilizing-transform TI Haar helps to elucidate the information of a complex biomedical sample. The instrument considerations and data analysis of Spectral-FLI are also discussed.

Original languageEnglish
Title of host publicationSpectroscopic Methods of Analysis
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Number of pages22
ISBN (Print)9781617798054
StatePublished - 1 Jan 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Chebyshev transform
  • FLI
  • FLIM
  • Fluorescence lifetime imaging microscopy
  • Polar plot
  • Spectral FLIM
  • TI Haar denoising

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