Fluorescence characteristics and lifetime images of photosensitizers of talaporfin sodium and sodium pheophorbide a in normal and cancer cells

Kamlesh Awasthi, Kazuhito Yamamoto, Kazunari Furuya, Takakazu Nakabayashi, Liming Li*, Nobuhiro Ohta

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Fluorescence spectra and fluorescence lifetime images of talaporfin sodium and sodium-pheophorbide a, which can be regarded as photosensitizers for photodynamic therapy, were measured in normal and cancer cells. The reduction of the fluorescence intensity by photoirradiation was observed for both photosensitizers in both cells, but the quenching rate was much faster in cancer cells than in normal cells. These results are explained in terms of the excessive generation of reactive oxygen species via photoexcitation of these photosensitizers in cancer cells. The fluorescence lifetimes of both photosensitizers in cancer cells are different from those in normal cells, which originates from the different intracellular environments around the photosensitizers between normal and cancer cells.

Original languageEnglish
Pages (from-to)11417-11430
Number of pages14
JournalSensors (Switzerland)
Volume15
Issue number5
DOIs
StatePublished - 18 May 2015

Keywords

  • Cancer cells
  • Fluorescence lifetime
  • Fluorescence spectrum
  • Photodynamic therapy
  • Reactive oxygen species
  • Sodium pheophorbide a
  • Talaporfin sodium

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