Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-γ expression

Shu Lin Chang, Yi Ming Chiang, Cicero Lee Tian Chang, Hsu Hua Yeh, Lie Fen Shyur, Yueh Hsiung Kuo, Tung-Kung Wu, Wen Chin Yang*

*Corresponding author for this work

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Bidens pilosa is used as an ethnical medicine for bacterial infection or immune modulation in Asia, America and Africa. Here, we employed an IFN-γ promoter-driven luciferase reporter construct and T cells to characterize immunomodulatory compounds from this plant based on a bioactivity-guided isolation principle. We found that PHA, a positive control, caused a six-fold increase in IFN-γ promoter activity. In contrast, hot water crude extracts from Bidens pilosa and its butanol subfraction increased IFN-γ promoter activity to two- and six-fold, respectively. Finally, centaurein (EC50 = 75 μg/ml) and its aglycone, centaureidin (EC50 = 0.9 μg/ml), isolated from this butanol subfraction, augmented IFN-γ promoter activity by ∼four-fold. Consistent with the role of centaurein or its aglycone in IFN-γ regulation, we showed that centaurein induced the activity of NFAT and NFκB enhancers, located within the IFN-γ promoter, in Jurkat cells. Overall, our results showed that centaurein regulated IFN-γ transcription, probably via NFAT and NFκB in T cells.

Original languageEnglish
Pages (from-to)232-236
Number of pages5
JournalJournal of Ethnopharmacology
Volume112
Issue number2
DOIs
StatePublished - 13 Jun 2007

Keywords

  • Bidens pilosa
  • Centaureidin
  • Centaurein
  • Flavonoid
  • Interferon gamma

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    Chang, S. L., Chiang, Y. M., Chang, C. L. T., Yeh, H. H., Shyur, L. F., Kuo, Y. H., Wu, T-K., & Yang, W. C. (2007). Flavonoids, centaurein and centaureidin, from Bidens pilosa, stimulate IFN-γ expression. Journal of Ethnopharmacology, 112(2), 232-236. https://doi.org/10.1016/j.jep.2007.03.001