Femtosecond laser processing of protein crystals in crystallization drop

Masafumi Kashii*, Hiroshi Kitano, Yoichiroh Hosokawa, Hiroaki Adachi, Yusuke Mori, Takatomo Sasaki, Hiroshi Masuhara, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Indue, Satoshi Murakami, Kazuomi Sugamoto, Hideki Yoshikawa

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


We have developed a novel processing technique for protein crystals using femtosecond laser irradiation. We call this technique the femtosecond-laser-induced cut and cleave operation (fs-CACO). By precisely controlling the laser fluence and the position of the laser focal point, we are able to perform accurate and reproducible processing of protein crystals with little damage without troublesome treatment such as the unsealing of vessels and removal of solutions surrounding the crystals. To our knowledge, this is the first report on processing protein crystals using ultra-short-pulse laser ablation. Fs-CACO will be a powerful tool for making problematic protein crystals suitable for X-ray diffraction measurements.

Original languageEnglish
JournalJapanese Journal of Applied Physics, Part 2: Letters
Issue number24-27
StatePublished - 24 Jun 2005


  • Femtosecond laser
  • Laser processing
  • Protein crystal
  • Structural analysis
  • X-ray diffraction

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