Femtosecond laser modification of living neuronal network

Chie Hosokawa*, Suguru N. Kudoh, Ai Kiyohara, Yoichiroh Hosokawa, Kazunori Okano, Hiroshi Masuhara, Takahisa Taguchi

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Scopus citations


An all-wet femtosecond laser microprocessing technique was utilized for patterning and cutting functional network of living neuronal cells on a multi-electrode dish (MED). The neuronal cells cultured on a source substrate were transferred onto an electrode in a MED probe in solution by utilizing a femtosecond laser-induced impulsive force and a pattern of neuronal cells were formed on the MED probe. The cellular activity of the detached neurons was supported that neurites could be regenerated around the electrodes. As another processing method, the neurons stretching between electrodes were selectively cut by the direct femtosecond laser irradiation and the spontaneous electrical activity of the neuronal network was evaluated. While the spontaneous action potentials of neurons were synchronized before the cutting, the synchronization disappeared after the cutting, indicating that the neuronal network is locally disconnected by the laser cutting. The present method is applicable to artificial reconstruction of living neuronal network.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalApplied Physics A: Materials Science and Processing
Issue number1
StatePublished - 1 Oct 2008

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