Femtosecond laser-induced crystallization of protein in gel medium

Kazuhiko Nakamura, Yosuke Sora, Hiroshi Y. Yoshikawa, Yoichiroh Hosokawa*, Ryota Murai, Hiroaki Adachi, Yusuke Mori, Takatomo Sasaki, Hiroshi Masuhara

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

We succeeded in generating femtosecond laser-induced crystallization of hen egg-white lysozyme (HEWL) in the irradiated area by suppressing convection of the solution using a highly concentrated gel. When a laser pulse, whose energy was above the threshold energy for bubble formation, was focused along with a linear scanning of the stage, HEWL crystallization was enhanced at the surface of bubbles on the irradiated line. The relation between the bubble formation process and the crystallization is discussed.

Original languageEnglish
Pages (from-to)6425-6429
Number of pages5
JournalApplied Surface Science
Volume253
Issue number15
DOIs
StatePublished - 30 May 2007

Keywords

  • Femtosecond laser
  • Hen egg-white lysozyme
  • Laser ablation
  • Light-induced crystallization
  • Protein crystal

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