Femtosecond laser-induced cleaving of protein crystal in water solution

Masafumi Kashii, Yoichiroh Hosokawa*, Hiroshi Kitano, Hiroaki Adachi, Yusuke Mori, Kazufumi Takano, Hiroyoshi Matsumura, Tsuyoshi Inoue, Satoshi Murakami, Kazuomi Sugamoto, Hideki Yoshikawa, Takatomo Sasaki, Hiroshi Masuhara

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

For precise X-ray diffraction (XRD) measurement giving the three-dimensional structure of proteins, it is important to prepare high-quality single crystals with suitable shape. As a new processing technique to obtain such protein crystals, we employed femtosecond laser-induced cleaving of protein crystal in a growth vessel containing water solution. An intact protein crystal was precisely processed without mechanical contact in its sealed growth vessel by focusing femtosecond laser pulses. We confirmed that three-dimensional processing of the crystal in its supersaturated solution was realized using multiphoton absorption and that the processing was efficiently enhanced by the cleaving behavior attributed to a photomechanical mechanism of the femtosecond laser ablation.

Original languageEnglish
Pages (from-to)6447-6450
Number of pages4
JournalApplied Surface Science
Volume253
Issue number15
DOIs
StatePublished - 30 May 2007

Keywords

  • Femtosecond laser
  • Laser processing
  • Photomechanical ablation
  • Photothermal ablation
  • Protein crystal
  • X-ray diffraction

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