Feasibility study of using refrigerant 1,1,1,2-tetrafluoroethane as the mobile phase in high-speed counter-current chromatography

Ya Ling Tsai, Tiing Yu*

*Corresponding author for this work

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Newly synthesized environmentally benign refrigerant 1,1,1,2-tetrafluoroethane (R134a) was used as the mobile phase in high-speed counter-current chromatography (HSCCC) in this study. R134a remains as a liquid under mild pressure and becomes a gas at atmospheric pressure. Accordingly, operation of HSCCC equipped with regular polytetrafluoroethylene tubing was satisfactory. Since R134a turned into gas at ambient temperature and pressure, further concentration for the collected fractions after elution was not required. A mixture of four standard components of Angelicae radix was separated to demonstrate the feasibility of using this new mobile phase in HSCCC. Copyright (C) 1999 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)237-242
Number of pages6
JournalJournal of Chromatography A
Volume835
Issue number1-2
DOIs
StatePublished - 12 Mar 1999

Keywords

  • Counter-current chromatography
  • Mobile phase composition
  • Tetrafluoroethane

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