Fabrication of PEG hydrogel microstructures in microfluidic system for multicellular spheroid formation, culturing and analysis

Chien Yu Fu*, Sheng Yang Tseng, Shih Mo Yang, Long Hsu, Cheng Hsien Liu, Hwan You Chang

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

This study presents a novel microfluidic device for 3-dimension cell culturing and analysis. Through defining the exposure to ultra-violet light by a photomask, localized photopolymerization of poly (ethylene glycol) diacrylate hydrogels form biocompatible and permselective microstructures within a microfluidic device. The designed geometry of microstructures allowed cells to be trapped into structures and to form spheroids in situ. Subsequent analysis and long-term three-dimension cell culture can achieve within the same device. This technique may apply as a microscale bioreactor or a platform for spheroid-based drug screening and study.

Original languageEnglish
Title of host publicationTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Pages98-100
Number of pages3
StatePublished - 23 Nov 2011
EventNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011 - Boston, MA, United States
Duration: 13 Jun 201116 Jun 2011

Publication series

NameTechnical Proceedings of the 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
Volume3

Conference

ConferenceNanotechnology 2011: Electronics, Devices, Fabrication, MEMS, Fluidics and Computational - 2011 NSTI Nanotechnology Conference and Expo, NSTI-Nanotech 2011
CountryUnited States
CityBoston, MA
Period13/06/1116/06/11

Keywords

  • Drug screening
  • Microfluidic systems
  • Microscale bioreactor
  • Multicellular spheroids
  • Photoresponsive hydrogel

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