TY - JOUR
T1 - Fabrication of microfluidic devices using dry film photoresist for microchip capillary electrophoresis
AU - Tsai, Yuan Chien
AU - Jen, Hsiu Ping
AU - Lin, Kuan Wen
AU - Hsieh, You-Zung
PY - 2006/4/14
Y1 - 2006/4/14
N2 - An inexpensive, disposable microfluidic device was fabricated from a dry film photoresist using a combination of photolithographic and hot roll lamination techniques. A microfluidic flow pattern was prefabricated in a dry film photoresist tape using traditional photolithographic methods. This tape became bonded to a poly(methyl methacrylate) (PMMA) sheet with prepouched holes when passed through a hot roll laminator. A copper working electrode and platinum decoupler was readily incorporated within this microchip. The integrated microchip device was then fixed in a laboratory-built Plexiglas holder prior to its use in microchip capillary electrophoresis. The performance of this device with amperometric detection for the separation of dopamine and catechol was examined. The separation was complete within 50 s at an applied potential of 200 V/cm. The relative standard deviations (RSD) of analyte migration times were less than 0.71%, and the theoretical plate numbers for dopamine and catechol were 3.2 × 10
4 and 4.1 × 10
4, respectively, based on a 65 mm separation channel.
AB - An inexpensive, disposable microfluidic device was fabricated from a dry film photoresist using a combination of photolithographic and hot roll lamination techniques. A microfluidic flow pattern was prefabricated in a dry film photoresist tape using traditional photolithographic methods. This tape became bonded to a poly(methyl methacrylate) (PMMA) sheet with prepouched holes when passed through a hot roll laminator. A copper working electrode and platinum decoupler was readily incorporated within this microchip. The integrated microchip device was then fixed in a laboratory-built Plexiglas holder prior to its use in microchip capillary electrophoresis. The performance of this device with amperometric detection for the separation of dopamine and catechol was examined. The separation was complete within 50 s at an applied potential of 200 V/cm. The relative standard deviations (RSD) of analyte migration times were less than 0.71%, and the theoretical plate numbers for dopamine and catechol were 3.2 × 10
4 and 4.1 × 10
4, respectively, based on a 65 mm separation channel.
KW - Amperometric detection
KW - Dry film photoresist
KW - Microchip capillary electrophoresis
KW - Microfluidic devices
UR - http://www.scopus.com/inward/record.url?scp=33645225789&partnerID=8YFLogxK
U2 - 10.1016/j.chroma.2005.12.003
DO - 10.1016/j.chroma.2005.12.003
M3 - Article
C2 - 16384565
AN - SCOPUS:33645225789
VL - 1111
SP - 267
EP - 271
JO - Journal of Chromatography A
JF - Journal of Chromatography A
SN - 0021-9673
IS - 2
ER -