Fabrication of microfluidic devices using dry film photoresist for microchip capillary electrophoresis

Yuan Chien Tsai, Hsiu Ping Jen, Kuan Wen Lin, You-Zung Hsieh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

An inexpensive, disposable microfluidic device was fabricated from a dry film photoresist using a combination of photolithographic and hot roll lamination techniques. A microfluidic flow pattern was prefabricated in a dry film photoresist tape using traditional photolithographic methods. This tape became bonded to a poly(methyl methacrylate) (PMMA) sheet with prepouched holes when passed through a hot roll laminator. A copper working electrode and platinum decoupler was readily incorporated within this microchip. The integrated microchip device was then fixed in a laboratory-built Plexiglas holder prior to its use in microchip capillary electrophoresis. The performance of this device with amperometric detection for the separation of dopamine and catechol was examined. The separation was complete within 50 s at an applied potential of 200 V/cm. The relative standard deviations (RSD) of analyte migration times were less than 0.71%, and the theoretical plate numbers for dopamine and catechol were 3.2 × 10 4 and 4.1 × 10 4, respectively, based on a 65 mm separation channel.

Original languageEnglish
Pages (from-to)267-271
Number of pages5
JournalJournal of Chromatography A
Volume1111
Issue number2
DOIs
StatePublished - 14 Apr 2006

Keywords

  • Amperometric detection
  • Dry film photoresist
  • Microchip capillary electrophoresis
  • Microfluidic devices

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