In this work, we fabricated gold-nanowires (Au NWs) on flexible substrates by direct current electrochemical deposition. Then, we electropolymerized a polypyrrole layer onto the Au NWs via cyclic voltammetry (CV) as the molecular imprinted polymer biosensor to detect dopamine (DA). The influence of the scan rate of CV for the sensor preparation was optimized. CV and differential pulse voltammetry (DPV) were used to measure different DA concentrations. The Au/polypyrrole core/shell nanowires sensor showed good DPV signal intensity at 805 μA mM-1 and the determined linear range for DA was 4 × 10-7 to 1 × 10-5 M (N = 3).