Fabrication of 3D microfluidic chip integrating concentration gradient design and perfusion system for embryo coculture with stromal cells

Pei Yu Chang, Kuo Wei Chang, Ciou Wen Lin, Po Chen Shih, Cheng Hsien Liu, Wen-Syang Hsu, Shih Kang Fan, Chin Jung Li, Chang Hung Tien, Hong Yuan Huang, Da Jeng Yao

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Fabrication of 3D chip that enables cell culture under continuous flow perfusion and repeated in situ observation by light microscopy is described. Thin polydimethylsiloxane PDMS) membrane was etched to create an array of through-holes, and sandwiched between two PDMS channels to form an upper chamber and lower chamber. Thus we fabricated a 3D microfluidic chip which integrates the concentration gradient design and perfusion system for proposed embryo coculture with stromal cells.

Original languageEnglish
Title of host publicationNANOMED 2012 - 6th IEEE International Conference on Nano/Molecular Medicine and Engineering
Pages32-35
Number of pages4
DOIs
StatePublished - 1 Dec 2012
Event6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012 - Bangkok, Thailand
Duration: 4 Nov 20127 Nov 2012

Publication series

NameIEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED
ISSN (Print)2159-6964
ISSN (Electronic)2159-6972

Conference

Conference6th IEEE International Conference on Nano/Molecular Medicine and Engineering, NANOMED 2012
CountryThailand
CityBangkok
Period4/11/127/11/12

Keywords

  • 3D chip
  • Mimetic uterus
  • Thin membrane

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