Fabrication and application of protein crystal microarrays

Yoichiroh Hosokawa, Satoshi Matsumura, Hiroshi Y. Yoshikawa, Hiroshi Masuhara*, Ryosuke Nakamura, Yasuo Kanematsu, Keiko Ikeda, Ai Shimo-Oka, Hajime Mori

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Scopus citations

Abstract

With the aim of fabricating highly integrated protein microarrays, functional proteins were occluded into a protein crystal, consisting of a proteinaceous occlusion body termed polyhedrin, and the crystals were individually fixed onto a polymer film by using laser trapping and bonding techniques. Individual crystals were trapped by the 1064 nm beam of a Nd3+:YAG laser and placed to the film. The bonding to the film was achieved by irradiating with the single shot of the 800 nm beam of a femtosecond Ti:sapphire laser. Enhanced green fluorescent protein (EGFP) was occluded into the polyhedrin crystal and the crystals were patterned and observed by florescence imaging and single crystal florescence spectroscopy. From these experiments, the biological activity of the bonded crystal was confirmed and their potential as element in protein microarrays was discussed.

Original languageEnglish
Title of host publicationBioinspired Nanoscale Hybrid Systems
Pages27-32
Number of pages6
Volume735
DOIs
StatePublished - 28 Jul 2003
EventBioinspired Nanoscale Hybrid Systems - Boston, MA, United States
Duration: 2 Dec 20024 Dec 2002

Publication series

NameMaterials Research Society Symposium - Proceedings
PublisherMaterials Research Society
ISSN (Print)0272-9172

Conference

ConferenceBioinspired Nanoscale Hybrid Systems
CountryUnited States
CityBoston, MA
Period2/12/024/12/02

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