Expression and secretion of hepatitis B viral surface antigen in E. coli

Yan-Hwa Wu Lee*, Yao Tsung Tung, Szecheng J. Lo

*Corresponding author for this work

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Hepatitis B viral surface antigen (HBsAg) gene was subcloned into the BglII site of Bacillus licheniformis penicillinase (penP) gene of secretory vector pJP104. Expression and secretion of HBsAg protein was achieved by the E. coli CS412 carrying the plasmid pJPS2 in which the penP:HBsAg hybrid gene was under the control of two promoters, lipoprotein (lpp) and penP, spaced 450 bases apart. The secreted form of HBsAg encoded by the hybrid penP:HBsAg gene of plasmid pJPS2 was purified by immunoaffinity chromatography and found to be a 25 kilodalton protein.

Original languageEnglish
Pages (from-to)1042-1049
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume135
Issue number3
DOIs
StatePublished - 28 Mar 1986

Fingerprint Dive into the research topics of 'Expression and secretion of hepatitis B viral surface antigen in E. coli'. Together they form a unique fingerprint.

  • Cite this