Expression and characterization of the τ subunit of phosphorylase kinase

L. R. Chen, Chiun-Jye Yuan, G. Somasekhar, P. Wejksnora, J. E. Peterson, A. M. Myers, L. Graves, P. T.W. Cohen, E. F. da Cruz e Silva, D. J. Graves*

*Corresponding author for this work

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A cDNA encoding the entire τ subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the Escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (CAT) gene, respectively. A significant phosphorylase kinase activity was found, in the first case. In the second case, a fused protein containing 73 amino acids from the CAT protein was obtained. After renaturation, the CAT-τ subunit protein shows enzymatic activity similar to the HPLC-purified and renatured τ subunit.

Original languageEnglish
Pages (from-to)746-753
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume161
Issue number2
DOIs
StatePublished - 15 Jun 1989

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