A cDNA encoding the entire τ subunit of rabbit skeletal muscle phosphorylase kinase was reconstructed and inserted into a plasmid containing the Escherichia coli ptac promoter and a constructed plasmid containing the ptac promoter and bacterial chloramphenicol acetyl transferase (CAT) gene, respectively. A significant phosphorylase kinase activity was found, in the first case. In the second case, a fused protein containing 73 amino acids from the CAT protein was obtained. After renaturation, the CAT-τ subunit protein shows enzymatic activity similar to the HPLC-purified and renatured τ subunit.
|Number of pages||8|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - 15 Jun 1989|