Abstract
A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.
| Original language | English |
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| Title of host publication | Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004 |
| Pages | 236-237 |
| Number of pages | 2 |
| DOIs | |
| State | Published - 1 Dec 2004 |
| Event | Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 - Taipei, Taiwan Duration: 15 Dec 2004 → 17 Dec 2004 |
Publication series
| Name | Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004 |
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Conference
| Conference | Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 |
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| Country | Taiwan |
| City | Taipei |
| Period | 15/12/04 → 17/12/04 |
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Cite this
Wei, P. K., Huang, P. C., & Chen, Y-C. (2004). Evanescent planar wave system for reading DNA microarrays on thin glass slides. In Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004 (pp. 236-237). (Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004). https://doi.org/10.1109/APBP.2004.1412369