Evanescent planar wave system for reading DNA microarrays on thin glass slides

Pei Kuen Wei; Peng Cheng Huang; Yi-Chun Chen

doi:10.1109/APBP.2004.1412369

Evanescent planar wave system for reading DNA microarrays on thin glass slides

Pei Kuen Wei^*, Peng Cheng Huang, Yi-Chun Chen

^*Corresponding author for this work

Institute of Imaging and Biomedical Photonics

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

2 Scopus citations

Abstract

A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.

Original language	English
Title of host publication	Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004
Pages	236-237
Number of pages	2
DOIs	https://doi.org/10.1109/APBP.2004.1412369
State	Published - 1 Dec 2004
Event	Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004 - Taipei, Taiwan Duration: 15 Dec 2004 → 17 Dec 2004

Publication series

Name	Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004

Conference

Conference	Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004
Country	Taiwan
City	Taipei
Period	15/12/04 → 17/12/04

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10.1109/APBP.2004.1412369

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title = "Evanescent planar wave system for reading DNA microarrays on thin glass slides",

abstract = "A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.",

author = "Wei, {Pei Kuen} and Huang, {Peng Cheng} and Yi-Chun Chen",

year = "2004",

month = dec,

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doi = "10.1109/APBP.2004.1412369",

language = "English",

isbn = "0780386760",

series = "Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004",

pages = "236--237",

booktitle = "Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004",

note = "null ; Conference date: 15-12-2004 Through 17-12-2004",

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Wei, PK, Huang, PC & Chen, Y-C 2004, Evanescent planar wave system for reading DNA microarrays on thin glass slides. in Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004. Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004, pp. 236-237, Second Asian and Pacific Rim Symposium on Biophotonics, APBP 2004, Taipei, Taiwan, 15/12/04. https://doi.org/10.1109/APBP.2004.1412369

Evanescent planar wave system for reading DNA microarrays on thin glass slides. / Wei, Pei Kuen; Huang, Peng Cheng; Chen, Yi-Chun.

Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004. 2004. p. 236-237 (Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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N2 - A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.

AB - A new optical reading system that can read the fluorescent signals of microarrays on glass slides with large detection area and high sensitivity is proposed. This system uses gratings (1200 g/mm) with several micron thick PDMS film on surface. Normally incident laser was coupled into a thin glass slide by grating coupling and confined in the slide due to the total internal reflection (TIR). The TIR generates evanescent planar wave on the glass surface. The evanescent wave was employed to excite the fluorescent signals in the microarrays. The method takes the advantages of low excitation background, large and uniform detection area.

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BT - Second Asian and Pacific Rim Symposium on Biophotonics - Proceedings, APBP 2004

Y2 - 15 December 2004 through 17 December 2004

ER -

Evanescent planar wave system for reading DNA microarrays on thin glass slides

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