Evaluation of immunomagnetic separation method for detection of Giardia for different reaction times and reaction volumes

B. M. Hsu, Ch-Hpin Huang*, Y. C. Chung, Y. C. Lai, H. S. Tai

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Immunomagnetic separation (IMS) has been specified as a standard method for the measurement of Giardia. In this study, Dynal IMS was evaluated on the basis of recovery efficiencies of Giardia cysts for various IMS operational conditions. The average recoveries for Giardia in deionized, treated and raw water samples were 82.6± 12.2% (n = 6), 75.6± 15.2% (n = 3), and 70.6 ±18.2% (n = 3), respectively. Significant changes in recovery were observed by altering the debris ratio and the debris components of water samples. Changing the reaction volume within the same vessel had no significant effect on cyst recovery efficiencies. However, prolonging the reaction time did increase recovery efficiencies.

Original languageEnglish
Pages (from-to)472-474
Number of pages3
JournalParasitology Research
Volume87
Issue number6
DOIs
StatePublished - 28 May 2001

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