Evaluation of carboxymethyl-hexanoyl chitosan as a protein nanocarrier

Mikael Larsson*, Annika Borde, Emma Mattisson, Dean-Mo LIu, Anette Larsson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Carboxymethyl-hexanoyl chitosan (CHC) has the ability to self-assemble into nanocapsules in an aqueous solution and it has recently shown potential in numerous biomedical applications. Here we investigate the protein loading efficiency and release, as well as the structural properties of CHC protein nanocarriers. Bovine serum albumin (BSA) or its chromophore labelled version, fluorescein-BSA, was used as a model protein and the loading was performed with a simple mixing of pre-formed nanocapsules and protein. Dynamic light scattering and zeta potential analysis revealed that protein loaded nanocarriers with high positive zeta potential were formed. The protein loaded nanocarriers displayed a loading efficiency of 75% and a very slow protein release. In summary, our results highlight the potential of CHC as a protein nanocarrier, but also indicate that protein-CHC interactions need to be considered in protein containing CHC formulations where protein release is not the main function.

Original languageEnglish
Article number7
JournalNanomaterials and Nanotechnology
Volume3
Issue number1
DOIs
StatePublished - 1 Jan 2013

Keywords

  • Amphiphilic chitosan
  • Carboxymethyl-hexanoyl chitosan
  • Nanocarrier
  • Nanoparticle
  • Protein delivery

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