Establishment and characterization of a human gastric carcinoma cell line TMC-1

Rong Yaun Shyu, Shun Yuan Jiang, Yiin Jeng Jong, Kuo Chen Cheng, Chi Hung Lin, Jyh Cherng Yu, Ming Fang Wu, Tzu Ming Chang*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Established cancer cell lines are useful in the study of various cancers. We established a human gastric carcinoma cell line TMC-1 derived from the lymph node of a moderately differentiated adenocarcinoma of the stomach. TMC-1 cells grew in vitro as a mixture of attached and suspension cells, and exhibited spindle or ovoid morphology. They had a population doubling time of 15 h, a plating efficiency of 61%, formed colonies in semisolid agar, secreted the tumor marker CA 19-9, and were tumorigenic in athymic nude mice. The cells expressed E-cadherin and β-catenin. The karyotypic analysis demonstrated hyperdiploid features with a modal chromosome of 53. The cell had the deletion at chromosome 18q and gains at chromosome 2p13-25, 5p15, 5q21-35, 7, 8q24, 9q, 11, 12p, 14q24-32 and 20. Analysis by fluorescence in situ hybridization showed the deletion at 7qtel and duplication at 7q11.2 at the rearranged chromosome 7. Growth of TMC-1 cells was inhibited by 27-32% by interferon-α (2,000 U/ml) and by interferon-γ with an IC 50 of 125 U/ml. The cell line is tumorigenic in vivo, and its growth is moderately inhibited by interferon-α and interferon-γ It can be used to develop new modalities of human gastric cancer treatment.

Original languageEnglish
Pages (from-to)37-46
Number of pages10
JournalCells Tissues Organs
Volume177
Issue number1
DOIs
StatePublished - 2004

Keywords

  • Cell line
  • Gastric cancer
  • Interferon
  • Karyotypic aberration

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