Epigenetic regulation of WNT3A enhancer during regeneration of injured cortical neurons

Chu Yuan Chang, Jui Hung Hung, Liang Wei Huang, Joye Li, Ka Shing Fung, Cheng Fu Kao, Linyi Chen*

*Corresponding author for this work

Research output: Contribution to journalArticle

Abstract

Traumatic brain injury is known to reprogram the epigenome. Chromatin immunoprecipitation-sequencing of histone H3 lysine 27 acetylation (H3K27ac) and tri-methylation of histone H3 at lysine 4 (H3K4me3) marks was performed to address the transcriptional regulation of candidate regeneration-associated genes. In this study, we identify a novel enhancer region for induced WNT3A transcription during regeneration of injured cortical neurons. We further demonstrated an increased mono-methylation of histone H3 at lysine 4 (H3K4me1) modification at this enhancer concomitant with a topological interaction between sub-regions of this enhancer and with promoter of WNT3A gene. Together, this study reports a novel mechanism for WNT3A gene transcription and reveals a potential therapeutic intervention for neuronal regeneration.

Original languageEnglish
Article number1891
Number of pages16
JournalInternational journal of molecular sciences
Volume21
Issue number5
DOIs
StatePublished - 10 Mar 2020

Keywords

  • Enhancer regulation
  • Epigenome
  • Histone modification
  • Neuronal regeneration
  • Transcriptional regulation
  • WNT3A

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