Enzyme-encapsulated silica nanoparticle for cancer chemotherapy

Yi Rong Chiu, Wei Jen Ho, Jiun Shuan Chao, Chiun-Jye Yuan*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

11 Scopus citations

Abstract

A novel horseradish peroxidase-encapsulated silica nanoparticle (SNP) was generated in this study under relatively mild conditions. The generated enzyme-encapsulated SNP were relatively uniform in size (average 70 ± 14.3 nm), monodispersed, and spherical, as characterized by transmission electron microscopy and scanning electron microscopy. The horseradish peroxidase encapsulated in silica nanoparticle exhibits biological properties, such as a pHdependent activity profile and km value, similar to that of free enzymes. Furthermore, enzyme-encapsulated SNP exhibited good operational stability for the repetitive usage with a relative standard deviation of 5.1 % (n = 10) and a high stability for long term storage (>60 days) at 4 °C. The feasibility of using enzyme-encapsulated SNP in prodrug cancer therapy was also demonstrated by its capability to convert the prodrug indole-3-acetic acid into cytotoxic peroxyl radicals and trigger the death of tumor cells. These results indicate that the developed enzyme-encapsulated SNP has potential in the applications of prodrug cancer therapy.

Original languageEnglish
Article number829
JournalJournal of Nanoparticle Research
Volume14
Issue number4
DOIs
StatePublished - 30 Mar 2012

Keywords

  • Enzyme encapsulation
  • HRP
  • IAA
  • Nanomedicine
  • Prodrug cancer therapy
  • Silicate nanoparticle

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