Enterotype-based Analysis of Gut Microbiota along the Conventional Adenoma-Carcinoma Colorectal Cancer Pathway

Tzu Wei Yang, Wei Hsiang Lee, Siang Jyun Tu, Wei Chih Huang, Hui Mei Chen, Ting Hsuan Sun, Ming Chang Tsai, Chi Chih Wang, Hsuan Yi Chen, Chi Chou Huang, Bei Hao Shiu, Tzu Ling Yang, Hsin Tzu Huang, Yu Pao Chou, Chih Hung Chou, Ya Rong Huang, Yi Run Sun, Chao Liang, Feng Mao Lin, Shinn-Ying HoWen Liang Chen, Shun Fa Yang, Kwo Chang Ueng, Hsien Da Huang, Chien Ning Huang, Yuh Jyh Jong, Chun Che Lin*

*Corresponding author for this work

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The dysbiosis of human gut microbiota is strongly associated with the development of colorectal cancer (CRC). The dysbiotic features of the transition from advanced polyp to early-stage CRC are largely unknown. We performed a 16S rRNA gene sequencing and enterotype-based gut microbiota analysis study. In addition to Bacteroides- and Prevotella-dominated enterotypes, we identified an Escherichia-dominated enterotype. We found that the dysbiotic features of CRC were dissimilar in overall samples and especially Escherichia-dominated enterotype. Besides a higher abundance of Fusobacterium, Enterococcus, and Aeromonas in all CRC faecal microbiota, we found that the most notable characteristic of CRC faecal microbiota was a decreased abundance of potential beneficial butyrate-producing bacteria. Notably, Oscillospira was depleted in the transition from advanced adenoma to stage 0 CRC, whereas Haemophilus was depleted in the transition from stage 0 to early-stage CRC. We further identified 7 different CAGs by analysing bacterial clusters. The abundance of microbiota in cluster 3 significantly increased in the CRC group, whereas that of cluster 5 decreased. The abundance of both cluster 5 and cluster 7 decreased in the Escherichia-dominated enterotype of the CRC group. We present the first enterotype-based faecal microbiota analysis. The gut microbiota of colorectal neoplasms can be influenced by its enterotype.

Original languageEnglish
Article number10923
JournalScientific reports
Volume9
Issue number1
DOIs
StatePublished - 1 Dec 2019

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    Yang, T. W., Lee, W. H., Tu, S. J., Huang, W. C., Chen, H. M., Sun, T. H., Tsai, M. C., Wang, C. C., Chen, H. Y., Huang, C. C., Shiu, B. H., Yang, T. L., Huang, H. T., Chou, Y. P., Chou, C. H., Huang, Y. R., Sun, Y. R., Liang, C., Lin, F. M., ... Lin, C. C. (2019). Enterotype-based Analysis of Gut Microbiota along the Conventional Adenoma-Carcinoma Colorectal Cancer Pathway. Scientific reports, 9(1), [10923]. https://doi.org/10.1038/s41598-019-45588-z