TY - JOUR
T1 - Enhancement of cell growth on honeycomb-structured polylactide surface using atmospheric-pressure plasma jet modification
AU - Cheng, Kuang Yao
AU - Chang, Chia Hsing
AU - Yang, Yi Wei
AU - Liao, Guo Chun
AU - Liu, Chih Tung
AU - Wu, Jong-Shinn
PY - 2017/2/1
Y1 - 2017/2/1
N2 - In this paper, we compare the cell growth results of NIH-3T3 and Neuro-2A cells over 72 h on flat and honeycomb structured PLA films without and with a two-step atmospheric-pressure nitrogen-based plasma jet treatment. We developed a fabrication system used for forming of a uniform honeycomb structure on PLA surface, which can produce two different pore sizes, 3–4 μm and 7–8 μm, of honeycomb pattern. We applied a previously developed nitrogen-based atmospheric-pressure dielectric barrier discharge (DBD) jet system to treat the PLA film without and with honeycomb structure. NIH-3T3 and a much smaller Neuro-2A cells were cultivated on the films under various surface conditions. The results show that the two-step plasma treatment in combination with a honeycomb structure can enhance cell growth on PLA film, should the cell size be not too smaller than the pore size of honeycomb structure, e.g., NIH-3T3. Otherwise, cell growth would be better on flat PLA film, e.g., Neuro-2A.
AB - In this paper, we compare the cell growth results of NIH-3T3 and Neuro-2A cells over 72 h on flat and honeycomb structured PLA films without and with a two-step atmospheric-pressure nitrogen-based plasma jet treatment. We developed a fabrication system used for forming of a uniform honeycomb structure on PLA surface, which can produce two different pore sizes, 3–4 μm and 7–8 μm, of honeycomb pattern. We applied a previously developed nitrogen-based atmospheric-pressure dielectric barrier discharge (DBD) jet system to treat the PLA film without and with honeycomb structure. NIH-3T3 and a much smaller Neuro-2A cells were cultivated on the films under various surface conditions. The results show that the two-step plasma treatment in combination with a honeycomb structure can enhance cell growth on PLA film, should the cell size be not too smaller than the pore size of honeycomb structure, e.g., NIH-3T3. Otherwise, cell growth would be better on flat PLA film, e.g., Neuro-2A.
KW - Atmospheric pressure plasma
KW - Cell proliferation
KW - Dielectric barrier discharge
KW - Honeycomb structure
KW - PLA
KW - Surface modification
UR - http://www.scopus.com/inward/record.url?scp=84994339717&partnerID=8YFLogxK
U2 - 10.1016/j.apsusc.2016.10.093
DO - 10.1016/j.apsusc.2016.10.093
M3 - Article
AN - SCOPUS:84994339717
VL - 394
SP - 534
EP - 542
JO - Applied Surface Science
JF - Applied Surface Science
SN - 0169-4332
ER -